Place the other equipment and supplies at the student work stations or in a central location where they can be located by group members and taken to their work stations. Set up the test tube racks with four test tubes per group. □ Methylene Blue (preferably in a dropping bottle) Pre Lab Serial Dilutions Equipment and materials for students The Pre Lab Exercise can be completed in forty five minutes. A student answer sheet and teacher Power Point are attached for use during this lab. This activity was adapted from An Introduction of Biotechnology developed by the Mathematics and Science Education Center, University of Missouri- St. The use of methylene blue gives them a visual model of how samples become less concentrated with each dilution step. The exercise will allow students to learn how to pipette, dilute samples and calculate the concentration of their samples. If students are not familiar with operating pipettes, the Pre Lab Serial Dilutions practice exercise is recommended. Pre Lab: Serial Dilutions Practice Exercise
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